Welcome to the Hayes laboratory website!
Our laboratory specializes in the investigation of base editing processes for the development of new tools to make specific changes to nucleic acid sequences. We are interested in the use of genetic engineering to help alleviate genetic disease in humans and the improvement of crops. If you are interested working in the laboratory please contact the PI through email about current openings.
Model organisms utilized include...
Bacterial expression models
Experience in the laboratory with the following plant models
- Arabidopsis thaliana
- Zea mays
- Ceratopteris richardii
- tomato
- tobacco
- Brachypodium distachyon
Techniques used include...
- Agrobacterial mediated transformation of Arabidopsis, tobacco, and tomato
- Tomato and tobacco tissue culture
- Plasmid design and cloning
- Recombinant protein expression and purification
- Enzymatic reactions and assay development
- ESI and MALDI-TOF mass spectrometry
- RT-PCR techniques
- ICP-MS and ICP-OES
- Sanger and Next Generation sequencing