Welcome to the Hayes laboratory website!

Dr. Hayes welcomes you

Our laboratory specializes in the investigation of base editing processes for the development of new tools to make specific changes to nucleic acid sequences. We are interested in the use of genetic engineering to help alleviate genetic disease in        humans and the improvement of crops. If you are interested working in the laboratory please contact the PI through email about current openings.

Model organisms utilized include...

Bacterial expression models

Cartoon of E. coli cells
E. coli

Experience in the laboratory with the following plant models

Showing a frond from a model fern species

  • Arabidopsis thaliana
  • Zea mays
  • Ceratopteris richardii
  • tomato
  • tobacco
  • Brachypodium distachyon

Techniques used include...

Plates of preconditioning tomato explants

 

 

  • Agrobacterial mediated transformation of Arabidopsis, tobacco, and tomato
  • Tomato and tobacco tissue culture
  • Plasmid design and cloning
  • Recombinant protein expression and purification
  • Enzymatic reactions and assay development
  • ESI and MALDI-TOF mass spectrometry
  • RT-PCR techniques
  • ICP-MS and ICP-OES  
  • Sanger and Next Generation sequencing